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2006•Unpublished venueRequires access

Study on Tissue Culture and Rapid Propagation ofAtractylodes macrocephala

HE Fu-ji

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Abstract

A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz..The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation.MS+BA 1.0 mg/L+NAA 0.3 mg/L+GA_3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also.MS+BA 3.0 mg/L+IBA 0.5 mg/L was suitable for the proliferation of axillary buds,with an induction rate of 95%.1/2MS+IBA 0.1~0.5 mg/L was optimum for rooting,with a rooting percentage more than 90%.The survival rate of transplanted plantlets was more than 90%.

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What this paper is about

A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz..The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation.MS+BA 1.0 mg/L+NAA 0.3 mg/L+GA_3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also.MS+BA 3.0 mg/L+IBA 0.5 mg/L was suitable for the proliferation of axillary buds,with an induction rate of 95%.1/2MS+IBA 0.1~0.5 mg/L was optimum for rooting,with a rooting percentage more than 90%.The survival rate of transplanted plantlets was more than 90%.

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Available abstract

A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz..The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation.MS+BA 1.0 mg/L+NAA 0.3 mg/L+GA_3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also.MS+BA 3.0 mg/L+IBA 0.5 mg/L was suitable for the proliferation of axillary buds,with an induction rate of 95%.1/2MS+IBA 0.1~0.5 mg/L was optimum for rooting,with a rooting percentage more than 90%.The survival rate of transplanted plantlets was more than 90%.

Key concepts: Petiole (insect anatomy), Callus, Explant culture, Tissue culture, Axillary bud, Botany, Horticulture, Biology

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