Study of apoptosis and Caspase-3,Fas expression in rat glioma after treatment with gamma knife

Objective To investigate the apoptosis and Caspase-3,Fas expression in rat glioma after treatment with gamma knife. Methods Setting up C6 glioma model with 60 rats,which were devided into a treatment group( n =30) and a control group( n =30). On the 14 th day after planting glioma cells,rats of the treatment group were subjected to gamma knife irradiation.At the 12 th hr,24 th hr,48 th hr,7 th day,14 th day,21 st day,flow cytometry was performed to estimate the glioma cells apoptosis and the expression of Caspase-3 and Fas.The relation between apoptosis and the two kinds of proteins was analysed. Results Compared with the control group,the apoptosis rate of the glioma cells in the treatment group increased obviously ( P 0.05),and at the 48 th hr reached its peak,then decreased gradually.The expression of Caspase-3 and Fas was positively correlated with apoptosis( r 1=0.928, r 2=0.916). Conclusion The apoptosis of the tumor cells is a kind of effect of gamma knife treatment.Caspase-3 and Fas gene may take part in the regulation of apoptosis.