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Study on the characteristics of polyphenol oxidase and peroxidase from banana peel

Yang Gong-ming

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Abstract

Taking catechol and guaiacol as substrates respectively,the characteristics of polyphenol oxidase (PPO) and peroxidase(POD) from banana peel were studied by spectrophotometer. The results showed that the optimum temperature and pH of PPO were 30℃ and 5.5 respectively, Km was 22.42mmol/L, Vmax was 0.027OD420/min. The PPO was inactivated at 75℃ for 5min and the extinct condition of PPO was 20s at 100℃. In addition, the PPO isoenzymes in its mixture. The optimum temperature and pH of POD were 30℃ and 6.0, the POD was inactivated at 80℃ for 5min and the extinct condition of POD was 25s at 100℃.

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What this paper is about

Taking catechol and guaiacol as substrates respectively,the characteristics of polyphenol oxidase (PPO) and peroxidase(POD) from banana peel were studied by spectrophotometer. The results showed that the optimum temperature and pH of PPO were 30℃ and 5.5 respectively, Km was 22.42mmol/L, Vmax was 0.027OD420/min. The PPO was inactivated at 75℃ for 5min and the extinct condition of PPO was 20s at 100℃. In addition, the PPO isoenzymes in its mixture. The optimum temperature and pH of POD were 30℃ and 6.0, the POD was inactivated at 80℃ for 5min and the extinct condition of POD was 25s at 100℃.

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Available abstract

Taking catechol and guaiacol as substrates respectively,the characteristics of polyphenol oxidase (PPO) and peroxidase(POD) from banana peel were studied by spectrophotometer. The results showed that the optimum temperature and pH of PPO were 30℃ and 5.5 respectively, Km was 22.42mmol/L, Vmax was 0.027OD420/min. The PPO was inactivated at 75℃ for 5min and the extinct condition of PPO was 20s at 100℃. In addition, the PPO isoenzymes in its mixture. The optimum temperature and pH of POD were 30℃ and 6.0, the POD was inactivated at 80℃ for 5min and the extinct condition of POD was 25s at 100℃.

Key concepts: Polyphenol oxidase, Guaiacol, Point of delivery, Peroxidase, Catechol, Banana peel, Catechol oxidase, Chemistry

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