Optimization Analysis of Slicing Methods to Mink Tissues by Frozen and Paraffin Section

Using frozen and paraffin section methods to the body tissues of minks,then HE staining was used for observation;comparing analyzed the effects of three different fixative in the frozen sections.The results showed that: Paraffin method was more suitable for brain tissue,and frozen section method was more suitable for skin tissue;the effects of three difference fixatives to frozen section methods,fixative A(anhydrous alcohol 80 mL、Formaldehyde 10% 10 mL,glacial acetic acid 10 mL) was better than fixative C(formalin 10%) and fixative B(Formaldehyde 40%15 mL, anhydrous alcohol 85 mL).