The Comparison of ELISA And ICA in Carcinoembryonic Antigen Detection

Objective:To compare magnetic particle capture immune luminescence(ICA) method and enzyme linked immunosorbent assay(ELISA) in determination of serum carcinoembryonic antigen (CEA) results,and understanding these two methods advantages and disadvantages and discuss ICA and ELISA detection of CEA sensitivity and accuracy.Methods:ICA and ELISA contrast test,recovery test and precision test.Results:Contrast test,Y was ICA method measured CEA content,X was ELISA measured CEA content,the regression equation:Y=1.32X+8.23,correlation coefficient r =0.991.Recovery test,with the ICA and ELSIA average recovery rate was 97.64% and 96.73%.The accuracy test,ELISA and the group of ICA in precision were 11.74% and 1.92% respectively. Batch precision was between 15.41% and 3.68% respectively.Conclusion:ICA and ELISA linear correlation is better,in the group,and group were superior to that of ELISA method between precision,ICA has high accuracy,stability is strong,ICA recovery than ELISA is high,the ICA in detection accuracy and stability compared with ELISA has a strong advantage.