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In Vivo Transformation of Mesenchymal Stem Cells (MSCs)in Rat Model of Spinal Cord Injury

Yubing Chen

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Abstract

Objective To observe the in vivo transformation of mesenchymal stem cells(MSCs)to neuron-like cells in rat model of spinal cord injury. Methods MSCs were proliferated and cultured in vitro, on which the expressions of surface markers CD34 and CD44 were determined by flow cytometry. The rat model of spinal cord injury was established by beating method, into which the Brdu-labeled MSCs were transplanted through tail vein. Twenty-one days after transplantation, the spinal cord tissues of model rats were isolated for immunohistochemical staining, and the expression of neurone specific enolase (NSE) was determined. Results No expression of CD34 but expression of CD44 was observed by flow cytometry in MSCs proliferated in vitro. Laser confocal microscopy showed NSE expression in the spinal cord tissues of rats transplanted with Brdu-labeled MSCs. Conclusion The transplanted MSCs may be migrated to injured spinal cord tissue and transformed to neuron-like cells.

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What this paper is about

Objective To observe the in vivo transformation of mesenchymal stem cells(MSCs)to neuron-like cells in rat model of spinal cord injury. Methods MSCs were proliferated and cultured in vitro, on which the expressions of surface markers CD34 and CD44 were determined by flow cytometry. The rat model of spinal cord injury was established by beating method, into which the Brdu-labeled MSCs were transplanted through tail vein. Twenty-one days after transplantation, the spinal cord tissues of model rats were isolated for immunohistochemical staining, and the expression of neurone specific enolase (NSE) was determined. Results No expression of CD34 but expression of CD44 was observed by flow cytometry in MSCs proliferated in vitro. Laser confocal microscopy showed NSE expression in the spinal cord tissues of rats transplanted with Brdu-labeled MSCs. Conclusion The transplanted MSCs may be migrated to injured spinal cord tissue and transformed to neuron-like cells.

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Available abstract

Objective To observe the in vivo transformation of mesenchymal stem cells(MSCs)to neuron-like cells in rat model of spinal cord injury. Methods MSCs were proliferated and cultured in vitro, on which the expressions of surface markers CD34 and CD44 were determined by flow cytometry. The rat model of spinal cord injury was established by beating method, into which the Brdu-labeled MSCs were transplanted through tail vein. Twenty-one days after transplantation, the spinal cord tissues of model rats were isolated for immunohistochemical staining, and the expression of neurone specific enolase (NSE) was determined. Results No expression of CD34 but expression of CD44 was observed by flow cytometry in MSCs proliferated in vitro. Laser confocal microscopy showed NSE expression in the spinal cord tissues of rats transplanted with Brdu-labeled MSCs. Conclusion The transplanted MSCs may be migrated to injured spinal cord tissue and transformed to neuron-like cells.

Key concepts: Mesenchymal stem cell, Spinal cord, Pathology, Enolase, CD34, CD44, Spinal cord injury, In vivo

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