Correlation analysis between hepatitis B virus DNA with HBeAg and ALT and its clinical significance

Objective To study the correlation between serum HBV DNA with e antigen-related quantitation and ALT levels in the patients with chronic hepatitis B(CHB) and its influence on diagnosis and prognosis.Methods The quantitative measurements of serum HBV DNA(with FQ-PCR) and HBeAg(with CLIA) were performed and ELISA was used for ALT detection in 1288 patients with CHB.Results Among the 1288 patients,the detection rates of HBV DNA in HBeAg positive and HBeAg negative patients were 75.3% and 24.8% respectively.The HBeAg quantity was significantly different in different HBV DNA loading groups:higher HBV DNA loading was associated with higher HBeAg quantity.The abnormal rate of ALT in HBeAg(+) group containing different HBVDNA had no difference in the magnitude(P 0.05),in HBeAg(-) group with HBV DNA contained in the order of the increase(P0.01).Conclusion The high positive rate and contents of HBV DNA in HBeAg positive patients further confirm that HBeAg is a reliable indicator for active HBV replication.The level of hepatocellular damage of HBV with HBeAg positive patients in serum is not correlated to the activity of virus reproduction,but the level of hepatocellular damage of HB with HBeAg negative patients is positively correlated to the load quantity of HBV.Combined detection of HBV DNA,ALT and HBeAg could be clinically most desirable.