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2010•Animal husbandry and feed scienceRequires access

Effects of Several Factors on Cryopreservation of Porcine Oocytes by Vitrification

Xuguang Liu

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Abstract

Cryopreservation can make the use of oocytes not constrained by time and space,and the cryopreserved oocytes can be used as materials for in vitro fertilization,nuclear transfer,transgene and other embryo engineering technology.Porcine oocytes are highly sensitive to lower temperatures due to rich lipid droplets in ooplasm.As a result,researches about cryopreservation of porcine oocytes get less progress.Vitrification is one of the effective methods for cryopreservation of oocytes.This paper discusses the effects of developmental stage of oocytes,cryopreservation carriers and cryoprotectant on cryopreservation of porcine oocytes by vitrification.

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Cryopreservation can make the use of oocytes not constrained by time and space,and the cryopreserved oocytes can be used as materials for in vitro fertilization,nuclear transfer,transgene and other embryo engineering technology.Porcine oocytes are highly sensitive to lower temperatures due to rich lipid droplets in ooplasm.As a result,researches about cryopreservation of porcine oocytes get less progress.Vitrification is one of the effective methods for cryopreservation of oocytes.This paper discusses the effects of developmental stage of oocytes,cryopreservation carriers and cryoprotectant on cryopreservation of porcine oocytes by vitrification.

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Available abstract

Cryopreservation can make the use of oocytes not constrained by time and space,and the cryopreserved oocytes can be used as materials for in vitro fertilization,nuclear transfer,transgene and other embryo engineering technology.Porcine oocytes are highly sensitive to lower temperatures due to rich lipid droplets in ooplasm.As a result,researches about cryopreservation of porcine oocytes get less progress.Vitrification is one of the effective methods for cryopreservation of oocytes.This paper discusses the effects of developmental stage of oocytes,cryopreservation carriers and cryoprotectant on cryopreservation of porcine oocytes by vitrification.

Key concepts: Vitrification, Cryopreservation, Cryoprotectant, Andrology, Human fertilization, Oocyte cryopreservation, Embryo, Biology

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