ScholarLens Back to search
2008•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Glucocapparin in Capparis spinosa L.

Mei Li-wan

Open publisher page 1 citations

Abstract

Objective To determine the content of glucocapparin in different parts of the Capparis spinosa L.collected from Turpan.Methods HPLC assay was applied as the quantitative analysis.Chromatographic conditions induded Phnomenex ODS(4.6 mm ×250 mm,5 μm) column and the mobile phase consisting of methanol/0.002mol·L-1 KCl solution(15∶85).The flow rate was 0.8 ml·min-1,and the detection wauelength was 224 nm.Results A good linear ranging of the glucocapparin was 1.96~58.8 μg·ml-1.Within the linear range showed good linear relation(r=0.999 9),Y=13 685.4X-109.1 and the average recoveries were 96.3%(RSD=2.0%).Conclusion This is simple and accurate to determine glucocapparin,which will be helpful to quality assessment of Capparis spinosa L.

About this research paper

What this paper is about

Objective To determine the content of glucocapparin in different parts of the Capparis spinosa L.collected from Turpan.Methods HPLC assay was applied as the quantitative analysis.Chromatographic conditions induded Phnomenex ODS(4.6 mm ×250 mm,5 μm) column and the mobile phase consisting of methanol/0.002mol·L-1 KCl solution(15∶85).The flow rate was 0.8 ml·min-1,and the detection wauelength was 224 nm.Results A good linear ranging of the glucocapparin was 1.96~58.8 μg·ml-1.Within the linear range showed good linear relation(r=0.999 9),Y=13 685.4X-109.1 and the average recoveries were 96.3%(RSD=2.0%).Conclusion This is simple and accurate to determine glucocapparin,which will be helpful to quality assessment of Capparis spinosa L.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To determine the content of glucocapparin in different parts of the Capparis spinosa L.collected from Turpan.Methods HPLC assay was applied as the quantitative analysis.Chromatographic conditions induded Phnomenex ODS(4.6 mm ×250 mm,5 μm) column and the mobile phase consisting of methanol/0.002mol·L-1 KCl solution(15∶85).The flow rate was 0.8 ml·min-1,and the detection wauelength was 224 nm.Results A good linear ranging of the glucocapparin was 1.96~58.8 μg·ml-1.Within the linear range showed good linear relation(r=0.999 9),Y=13 685.4X-109.1 and the average recoveries were 96.3%(RSD=2.0%).Conclusion This is simple and accurate to determine glucocapparin,which will be helpful to quality assessment of Capparis spinosa L.

Key concepts: Capparis spinosa, Chromatography, Linear relationship, Chemistry, Linear range, High-performance liquid chromatography, Traditional medicine, Detection limit

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Glucocapparin in Capparis spinosa L. — Research Paper | ScholarLens