Optimizing agroinfiltration-mediated transient expression in Nicotiana benthamiana using pCB302-3 vector

pCB302-3is a plant mini-binary stable transformation vector.To investigate the transient expression of pCB302-3vector in plant,GFP was inserted into pCB302-3vector as a reporter gene,and various factors including density of Agrobacterium cell,supplementation of gene silencing suppression p19 and days post infiltration were optimized based on agroinfiltration method in Nicotiana benthamiana leaves.Results showed that high levels of GFP expression were observed in N.benthamianaleaves 3-5d after infiltration by Agrobacterium cell suspension contained pCB302-3-GFP with an optical density(D600)of 0.8-1.0co-infiltrated with p19 gene.