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2011•Guizhou nongye kexueRequires access

Optimization of Alkaloids Extraction Conditions from Coptis chinensis by Complex Enzyme Method

Yang Zheng-shui

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Abstract

The optimum extraction technological condition of alkaloids from C.chinensis by complex enzyme method was studied by determining alkaloids content in C.chinensis using Reversed-Phase HPLC.The results showed that the optimum extraction conditions included 1∶0.3 of cellulase∶pectinase,pH 4.5 and 45℃ for 3h.The alkaloids extraction rate of the complex enzyme method from C.chinensis was higher than the traditional extraction method.

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The optimum extraction technological condition of alkaloids from C.chinensis by complex enzyme method was studied by determining alkaloids content in C.chinensis using Reversed-Phase HPLC.The results showed that the optimum extraction conditions included 1∶0.3 of cellulase∶pectinase,pH 4.5 and 45℃ for 3h.The alkaloids extraction rate of the complex enzyme method from C.chinensis was higher than the traditional extraction method.

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Available abstract

The optimum extraction technological condition of alkaloids from C.chinensis by complex enzyme method was studied by determining alkaloids content in C.chinensis using Reversed-Phase HPLC.The results showed that the optimum extraction conditions included 1∶0.3 of cellulase∶pectinase,pH 4.5 and 45℃ for 3h.The alkaloids extraction rate of the complex enzyme method from C.chinensis was higher than the traditional extraction method.

Key concepts: Pectinase, Cellulase, Extraction (chemistry), Coptis chinensis, Chromatography, Coptis, Chemistry, Enzyme

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