Damage of Bisphenol A on Reproductive System and Effects the Expression of Bax and Bcl-2 Genes Induced in Male Rats

20 male SD rats were divided into 2 groups randomly including normal saline and Bisphenol A(BPA) group.The BPA group rats were export to BPA by intragastric administration at dose of 5.00 mg/kg for 4 weeks.After exposure,the body weight,testis wet weights,ratios of testis/body weight were determined.The histological observation of the testis and epididymis was carried out.The expression of Bcl-2 and Bax in rat spermatogenic cells were measured by immunohistochemical method.Compared with the control group,testis wet weights,the ratio of testis/weight obviously reduced(P0.01).Optical microscopy showed the number of spermatozoon,spermatocyte and sperm cell was all reduced,the number of necrosis cells in testis and epididymis was increased,and the number of sperms in the epididymal duct were reduced.Meanwhile,Bcl-2 expression was significantly decreased while Bax expression was increased in BPA exposure groups(P0.01).The ratio of Bcl-2/Bax was remarkably reduced.The results suggested that BPA could damage the reproductive system of male mice.The mechanism is that BPA induced necrosis of the germ cell in testis and epididymis,and downregulated expression of Bcl-2,upregulated expression of Bax of germ cell.