Effects of ulinastatin on cellular immunity and hepatorenal functions in patients with laparoscopic surgery for colorectal cancer

Objective To investigate the effects of ulinastatin on cellular immunity and hepatorenal functions in patients with laparoscopic surgery for colorectal cancer.Methods Sixty patients with laparoscopic colorectal cancer surgery(ASA Ⅰ or Ⅱ,aged 32~78 year,weighing 42~77 kg),were divided into Group Ⅰ,Ⅱ,Ⅲ and Group C.Before anesthesia induction,ulinastatin of 0.5×104,1.0×104 and 1.5×104 U/kg,and the replaced saline were given to the subjects in Group Ⅰ,Ⅱ,Ⅲ and C,respectively.Venous blood samples were collected for assessment of T-lymphocyte subsets(CD3+,CD4+,CD8+ and CD4+/CD8+),liver function(AST and ALT) and renal function(BUN and Cr) before anesthesia induction(T0),on the first day(T1),the third day(T2),the fifth day(T3) and the seventh day(T4).Results No significant difference was revealed in CD3+,CD4+,CD8+ or CD4+/CD8+ among the four groups at T0(P0.05).Compared to T0,CD3+ and CD4+ in Group C,CD4+ in Group Ⅰ,Ⅱ and Ⅲ at T1,and CD3+,CD4+ and CD4+/CD8+ in Group C at T2 were significantly reduced(P0.05).However,CD3+,CD4+ and CD4+/CD8+ were significantly reversed at T2 in Group Ⅰ,Ⅱ and Ⅲ.CD3+ at T2,and CD4+ at T2 and T3 in Group Ⅰ and Ⅱ;CD3+,CD4+ and CD4+/CD8+ at T2 and T3 in Group Ⅲ were significantly higher than those in Group C(P0.05).There was no significant difference in CD3+,CD4+,or CD4+/CD8+ among Group Ⅰ,Ⅱ,Ⅲ;neither was in CD8+,AST,ALT,BUN or Cr among the four groups(P0.05).Conclusion The cellular immune function is reduced 1~3 days after laparoscopic surgery for colorectal cancer,which can be alleviated by ulinastatin(most effective in 1.5×104 U/kg) with no effect on hepatorenal functions.