Activity analysis of Cry protein from Bacillus thuringinesis against Helicoverpa armigera
Zhao KuiJun
Abstract
Zhao KuiJun
Abstract
The bioassay was done by using 6 Bt Cry proteins(Cry2Ab4,Cry1Ia8,Cry1Ie1,Cry1Ca7,Cry1Cb2) and protein(Cry1Ac) from a natural Bt strain(HD-73) and combination of Cry1Ac proteins and the other 5 Bt Cry proteins by 1:1 against Helicoverpa armigera.The results showed that Cry1Ac had higher toxicity than other Cry proteins,the LC50 of Cry1Ac was 3.16 μg·mL-1,followed by Cry2Ab4.The co-toxicity coefficient LC50 of Cry1Ac and Cry2Ab4 combination was 48.70 μg·mL-1 and The co-toxicity coefficient of Cry1Ac and Cry2Ab4 was 1.21,which showed that the two kinds of proteins could synergize each other.Combining Cry1Ac and the other 5 Cry proteins had high toxicity to H.armigera.
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The bioassay was done by using 6 Bt Cry proteins(Cry2Ab4,Cry1Ia8,Cry1Ie1,Cry1Ca7,Cry1Cb2) and protein(Cry1Ac) from a natural Bt strain(HD-73) and combination of Cry1Ac proteins and the other 5 Bt Cry proteins by 1:1 against Helicoverpa armigera.The results showed that Cry1Ac had higher toxicity than other Cry proteins,the LC50 of Cry1Ac was 3.16 μg·mL-1,followed by Cry2Ab4.The co-toxicity coefficient LC50 of Cry1Ac and Cry2Ab4 combination was 48.70 μg·mL-1 and The co-toxicity coefficient of Cry1Ac and Cry2Ab4 was 1.21,which showed that the two kinds of proteins could synergize each other.Combining Cry1Ac and the other 5 Cry proteins had high toxicity to H.armigera.
Key concepts: Cry1Ac, Helicoverpa armigera, Bioassay, Bacillus thuringiensis, Toxicity, Biology, Toxicology, Chemistry