Promoter methylation of RASSF1A gene in exfoliated cervical cytology from cervical carcinoma and its clinical application

Objective To detect promoter methylation of Ras association domain family 1A(RASSF1A) gene in exfoliated cervical cytology in cervical carcinoma and study its clinical application.Methods Methylation specific polymerase chain reaction was applied to detect RASSF1A gene promoter′s methylation in exfoliated cervical cytology from cervical carcinoma patients and healthy females.The relationship between clinicopathological factors and RASSF1A gene promoter′s methylation was analyzed.Results The methylation rate in exfoliated cervical cytology from cervical carcinoma patients was 64.0%,higher than 1.3% in healthy females(P0.05).The differences of RASSF1A gene′s methylation rates in histology staging,tumor diameters,lymph node metastasis,metastasis and Figo staging were statistics significant(P0.05).Conclusion Methylation of RASSF1A gene promoter could be served as a tumor biomarker for cervical carcinoma in disease condition and prognosis evaluation.