siRNA inhibits vegf expression in bladder cancer

Objective To investigate the inhibition effect of the RNA interfere targeting VEGF on the growth of bladder cancer cell in vitro and in vivo.Methods The siRNA eukaryotic expression vector targeting VEGF gene,named PsilencerTM-VEGF-siRNA was constructed and transfected into T24 cell.The inhibition effects on VEGF gene were determined by reverse transcription PCR analysis.The invitro cellular growth activities were assayed by MTT colorimetry.The cell apoptosis was studied boflow cytometry.TO observed the therapeutic effects of siRNA VEGF through xenograft tumor nude mice model.VEGF expression was analyze with RT-PCR and Western blot.Results After the siVEGF transfected into the T24 cells,the expressio of VEGF gene was inhibited significantly(by 79%).The cellular growth activities in the T24 cells transfected with siVEGF decreased obviously,significantly lower than those in the regative control group and in the nontransfected group(P0.01).Significantly apoptosis in the T24 cells transfected with siVEGF was induced.The growth speed and formation rate of xenograft tumor in siRNA VEGF transfected mice slowed down significantly,and VEGF expression were down regulated too.However,there was no similar inhibitive effect in the control groups.Conclusion siRNA targeting VEGF gene inhibit VEGF expression and inhibits the growth of bladder cancer.