The model construction of partial orthotopic liver transplantation with hepatic arterialreconstruction in rats and its significance

Objective:To investigate the surgical procedures of hepatic arterial reconstruction in partial orthotopic liver transplan-tation(OLTs) in rats and comparatively study its significance. Methods:One hundred and twenty SD rats were randomly divided into 2 groups: Partial orthotopic liver transplantation group with hepatic arterial reconstruction(test group) and partial orthotopic liver transplantation group without hepatic arterial reconstruction(control group). Liver function, histological findings and proliferate index(PI) by flow cytometry were detected on the 1st, 2nd, 4th and 7th day after surgery respectively. Results:Alanine aminotransferase(ALT) and total bilirubin(TB) began to elevate from the first day after surgery. ALT in the control group on the fourth day and seventh day were significantly higher than those in the test group(P 0.05). TB in the control group at the fourth day were significantly higher than those in the test group(P 0.05). Histological findings indicated that more diploid and polyploid hepatocytes could be seen in the test group. PI in the test group on the second and fourth day were significantly higher than those in the control group[(32.81 ± 3.45)%,(36.33 ± 2.52)% vs(24.08 ± 4.66)%,(29.79 ± 1.82)%]. Conclusion:Hepatic arterial reconstruction can significantly improve liver function of liver grafts after POLT, and promote the regeneration of liver grafts.