2001•Zhongguo renshougonghuanbing zazhiRequires access

DISCOVERY OF SEVERAL GENES ASSOCIATED WITH ENZYME OF SCHISTOSOMA JAPONICUM (CHINEASE STRAIN)

Xiao Shao

Abstract

Aim To isolate and identify Schistosoma japonicum(Chinese strain)valuable genes from a directional cDNA library by EST strategy.Methods The cDNA clones were selected randomly,and the ESTs were obtained by PCR directed sequencing,then the novel ESTs were submitted to NCBI dbEST and GenBank for obtaining accession numbers.Results 362 recombinant clones picked out of the cDNA library,and the inserts of cDNA clones were identified by PCR.90 ESTs were generated by PCR directed sequencing.Among them,7 genes associated with enzymes were studied further.Conclusion This study shows that EST strategy can be used to discover novel valuable genes of Schistosoma japonicum.

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Key concepts: Schistosoma japonicum, Biology, GenBank, cDNA library, Expressed sequence tag, Complementary DNA, Gene, Accession number (library science)