Construction and Preliminary Identification of Eukaryotic Expression Vector of Cryptosporidium parvum miR-2980

[Objective] This study aimed to construct and preliminarily identify the eukaryotic expression vector of C.parvum miR-2980.[Method] The cp-miR-2980 precursor was amplified from C.parvum genomic DNA and cloned into pMD18-T vector.The amplified precursor was then subcloned into pVAXⅠvector and identified with restriction endonuclease digestion and sequencing.The recombinant plasmid pVAX-miR2980 was transfected into HCT-8 cells.Total RNA was extracted and the expression of cp-miR-2980 was evaluated by RT-PCR detection.[Result] The results showed that the recombinant eukaryotic expression vector pVAX-miR2980 was successfully constructed,which can express cp-miR-2980 in HCT-8 cell.[Conclusion] This study laid the foundation for further exploring the biological function of cp-miR-2980.