STUDY ON THE p 53 GENE IN HUMAN HEPATOMA CELL LINE QGY-7703 AND ITS EXPRESSION

p 53 gene in human hepatoma cell line QGY-7703 was studied wjth following methods: 17 p alleles analysis, PCR-SSCP, RT-PCR/sequencing, Northern blot, immunohistochemistry, Western blot and immunoprecipitation. There may exist allelic loss in the vicinity of p 53 gene on chromosome 17 p. Whereas no any point mutations were found in the coding region of p 53 gene in the cell, Northern blot and immunoprecipitation experiments showed its expre-ssion is quite low, which may be relative with the malignancy of the cell.