Effect of platelet-derived growth factor on the expressions of CD44 and β-catenin in vascular smooth muscle cell of rats

Objective To investigate the effects of platelet-derived growth factor(PDGF)on the expressions of CD44 and β-catenin in vascular smooth muscle cell of rats,and to explore the influence of CD44 and β-catenin on cell proliferation and migration of vascular smooth muscle cells(VSMCs).Methods The primary culture VSMCs of rats were treated by PDGF,then the cell proliferation of VSMCs at different time points was detected by MTT.The cell migration condition of VSMCs treated by PDGF was detected by cell migration test,and the changes of expression of CD44 and β-catenin of VSMCs at 0min,20min,6h after stimulated by PDGF were detected by gene chips and RT-PCR.Results The proliferation activity of VSMCs treated by PDGF at 6h,12h,24h was as 1.44,2.14,3.05 times as that in control group(0min),respectively,which suggested that PDGF obviously promoted the proliferation of VSMCs(P0.05).The examination results of cell migration test showed that the cell migration activity of VSMCs 20min,6h after treated by PDGF was as 1.58 and 3.37 times as that in control group,which suggested that the cell migration activity of VSMCs was significantly increased.The results of gene chips screening and RT-PCR confirmed further that the expression of CD44 of VSMCs 20min,6h after treated by PDGF was as 3.1 and 5.0 times as that in control group(P0.05),which suggested that PDGF had obvious induction effect on the expression of β-catenin of VSMCs,and the gene expression 20min,6h after exposure to PDGF was as 3.77 and 5.46 times as that in control group(P0.05).Conclusion PDGF can enhance the expression of CD44 and β-catenin of VSMCs in a shorter time.meanwhile,PDGF can significantly promote the proliferation and migration of VSMCs.