Multiplex PCR for detection of six species of foodborne pathogens

Objective To develop an effective method for detection of foodborne pathogens and diagnosis of food-poisoning.Methods Six species of foodborne pathogens,i.e.,E.coli-ETEC (Enterotoxigenic Escherichia coli) ,Bacillus cereus,Salmonella spp,E.coli-O157:H7,Shigella spp,Vibrio cholerae,were specifically detected in one multiplex PCR system.Results A method with high sensitivity and specificity was developed for the rapid detection and identification of specific pathogenic bacteria from various food materials.Conclusions The sensitivity of multiplex PCR corresponded to that of single PCR,that means both attain the same lowest detectable level of 10.30 fg.A gene diagnostic kit for food testing and diagnosis of food-poisoning will be developed by the multiplex PCR method.