Expression of porcine marrow mesenchymal stem cells transfected by lentiviral vector-meadiated HO-1 gene

Objective To investigate the feasibility of recombinant heme oxygenase-1(HO-1) gene-transfected porcine mesenchymal stem cells(MSCs) in vetro in gene tharapy.Methods Lentiviral system was utilized to introduce HO-1 gene into MSCs isolated from porcine bone marrow and cultured in vitro.RT-PCR and GFP marker were used to determine the expression of HO-1.MTT and trypan blue staining were used to detect the proliferative capacity of the MSCs.Results MSCs were infected with lentivirus at a multiplicity of infection(MO I) of 20 with optimal expression efficiency of 80%.The expressions of CD44 and CD105 on surface of MSCs were observed at high levels.GFP marker was observed at 96 hours after gene transfection and then gradually enhanced.The expression of HO-1 mRNA appeared in the transfected cells.HO-1 transfection did not show any significantly inhibitory effect on MSCs.Conclusion The expression of HO-1 is up-regulated in MSCs transfected successfully by lentiviral vector and the transfection has no significant effects on the survival and proliferation of MSCs.