MUTAGENESIS OF GENE-TRANSDUCED CELL TCA8113/TNF-α IN VITRO

Objective To investigate the safety of gene transduced Tca8113/TNF α as tumor vaccine. Methods TNF α gene was transduced into Tca8113. The mutagenesis of the DNA and supermatant of Tca8113/TNF α in vitro was tested by means of genetic toxicological techniques — the Ames assays. Results (1)?The hightest number (x±s) of the bacteria clone of the DNA groups were 150 5±11 2/160 2±7 6 (TA97); 34 0±2 2/34 4±3 2 (TA98); 139 8±4 6/130 8±7 2 (TA100); 264 0±14 6/254 4±8 2 (TA102)。(2)?The highest number (x±s) of the bacteria clone of the supernatant groups were 140 5±11 2/155 2±7 6 (TA97); 34 0±1 0/36 4±7 2 (TA98); 145 6±5 6/164 4±6 2 (TA100); 254 0±6 0/284 4±8 2 (TA102)。 Conclusion The mutagenesis of both the DNA and supermatant of transgenic cell Tca8113/TNF α was not observed. The initial experiment suggested that the application of gene transduced cell Tca8113/TNF α as tumor vaccine was basically safe and reliable.