2010•Chinese Journal of Gastroenterology and HepatologyRequires access
Apoptosis of hepatoma carcinoma cells induced by Trail with Survivin promoter
Cheng Tianming
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Abstract
Objective To investigate the expression difference of Trail protein induced by Survivin promoter in different cells and to evaluate the apoptosis of hepatoma carcinoma cells induced by Trail with Survivin promoter.Methods Using pGL3-surp340 plasmid as template,Survivin340 gene promoter was amplified.The recombinant plasmid pcDNA3.1/SurP/Trail was constructed.The expression differenece of Trail protein between HepG2 cells and 3T3 cells was detected by Western Blotting method.The apoptosis of HepG2 cells and 3T3 cells which transfected with pcDNA3.1/SurP/Trail was analyzed by double labeling flow cytometry.Results The recombinant plasmid pcDNA3.1/SurP/Trail was successfully constructed.Western Blotting results showed that the expression of Trail protein in HepG2 cells(0.49±0.43) was higher than that in 3T3 cells(0.15±0.37),and the apoptosis rate of HepG2 cells transfected with recombinant plasmid(11.59±0.74) was also significantly higher than other groups(P0.05).Conclusion The recombinant plasmid pcDNA3.1/SurP/Trail containing Survivin promoter enhanced the expression of Trail in tumor cells and induced cell apoptosis of HepG2 cells.