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2002•Xumu shouyi xuebaoRequires access

Sequencing for the New Alleles of Chicken Growth Hormone Gene Intron 4

Qinghua Nie, Xiquan Zhang, Yang Guanfu, Fcc Leung

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Abstract

Intron 4 polymorphisms of cGH in different Chinese native chicken breeds(lines)and other breeds were determined and the new alleles were sequenced in the present study.The results showed that intron 4 was 1031-1200 bp long and that alleles A?B?C?D was 1170 bp?1164 bp?1168 bp and 1166 bp long,of which some base pairs were different.The change of the 580th base pair(A→G)and the 688th base pair(A→G)produced two sites of restriction enzyme MspⅠ,which was the causes of polymorphisms of cGH gene intron 4.Two-band PCR productwas produced by 51 bp fragments deleted in position 420～470 of one chain.The sequencing results were consistent with those the electrophoresis showed.

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What this paper is about

Intron 4 polymorphisms of cGH in different Chinese native chicken breeds(lines)and other breeds were determined and the new alleles were sequenced in the present study.The results showed that intron 4 was 1031-1200 bp long and that alleles A?B?C?D was 1170 bp?1164 bp?1168 bp and 1166 bp long,of which some base pairs were different.The change of the 580th base pair(A→G)and the 688th base pair(A→G)produced two sites of restriction enzyme MspⅠ,which was the causes of polymorphisms of cGH gene intron 4.Two-band PCR productwas produced by 51 bp fragments deleted in position 420～470 of one chain.The sequencing results were consistent with those the electrophoresis showed.

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Available abstract

Intron 4 polymorphisms of cGH in different Chinese native chicken breeds(lines)and other breeds were determined and the new alleles were sequenced in the present study.The results showed that intron 4 was 1031-1200 bp long and that alleles A?B?C?D was 1170 bp?1164 bp?1168 bp and 1166 bp long,of which some base pairs were different.The change of the 580th base pair(A→G)and the 688th base pair(A→G)produced two sites of restriction enzyme MspⅠ,which was the causes of polymorphisms of cGH gene intron 4.Two-band PCR productwas produced by 51 bp fragments deleted in position 420～470 of one chain.The sequencing results were consistent with those the electrophoresis showed.

Key concepts: Biology, Intron, Allele, Genetics, Gene, Base pair, Restriction site, Polymerase chain reaction

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Sequencing for the New Alleles of Chicken Growth Hormone Gene Intron 4 — Research Paper | ScholarLens