THE EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR AND DEXAMETHASONE ON PROLIFERATION AND DIFFERENTIATION OF BONE MARROW STROMAL CELLS IN SKELETAL SYSTEM

Objective To investigate the effects of basic fibroblast grwoth factor and dexamethasone on proliferation and differentiation of bone marrow stromal cells. Methods After treatment with different concentrations of basic fibroblast growth factor and dexamethasone,cell proliferation and cell differentation were measured. Results 100?μg/L bFGF could promote cell proliferation significantly,but the level of ALP was the lowest at the same time.Dexamethasone inhibited cell proliferation.With the increase of concentration of dexamethasone the effect was enhanced.At the same time dexamethasone promoted the activity of ALP.This effect was enhanced with the increase of concentration of dexamethasone.The activity of ALP was enhanced 2 to 4 fold with the dexamethasone for 6 days.Conclusion bFGF promoted the proliferation and inhibited the differentiation of bone marrow stromal cells.Dexamethasone inhabited the proliferation of bone marrow stromal cells,while induced them to differentiate into osteoblasts.The appropriate concentration of dexamethasone was 10 -8 ?mol/L.\;[