ScholarLens Back to search
2005•Journal of Tropical MedicineRequires access

Inhibition of HBsAg and HBeAg Expression by ASODN of Various Regions of the HBV Genome

Jun Tang

Open publisher page 0 citations

Abstract

Objective To study the effect of antisense phosphorothioate oligodeoxynucleotides (S-as ODN) of various regions of the HBV genome on the expression of HBsAg and HBeAg in the HepG22215 cell line. Method The expression levels of HBsAg and HBeAg in the HepG22215 cell line were determined by ELISA. According to gene structure of HBV, 5 S-as ODNs were designed and synthesized. The effect of different ASODNs on the expression of HBsAg and HBeAg was analyzed. Results This confirmed the characteristics of HepG22215 which could produce HBsAg and HBeAg. All 5 S-as ODNs could specifically inhibit the production of HBsAg and HBeAg in HepG22215 cells. A combination of drug and ASODN could enhance the inhibition. Conclusion These results suggest that S-as ODN may be a potential new therapy for anti-HBV. The inhibition showed sequence-specific, dose-dependent and drug-cooperative effects.

About this research paper

What this paper is about

Objective To study the effect of antisense phosphorothioate oligodeoxynucleotides (S-as ODN) of various regions of the HBV genome on the expression of HBsAg and HBeAg in the HepG22215 cell line. Method The expression levels of HBsAg and HBeAg in the HepG22215 cell line were determined by ELISA. According to gene structure of HBV, 5 S-as ODNs were designed and synthesized. The effect of different ASODNs on the expression of HBsAg and HBeAg was analyzed. Results This confirmed the characteristics of HepG22215 which could produce HBsAg and HBeAg. All 5 S-as ODNs could specifically inhibit the production of HBsAg and HBeAg in HepG22215 cells. A combination of drug and ASODN could enhance the inhibition. Conclusion These results suggest that S-as ODN may be a potential new therapy for anti-HBV. The inhibition showed sequence-specific, dose-dependent and drug-cooperative effects.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To study the effect of antisense phosphorothioate oligodeoxynucleotides (S-as ODN) of various regions of the HBV genome on the expression of HBsAg and HBeAg in the HepG22215 cell line. Method The expression levels of HBsAg and HBeAg in the HepG22215 cell line were determined by ELISA. According to gene structure of HBV, 5 S-as ODNs were designed and synthesized. The effect of different ASODNs on the expression of HBsAg and HBeAg was analyzed. Results This confirmed the characteristics of HepG22215 which could produce HBsAg and HBeAg. All 5 S-as ODNs could specifically inhibit the production of HBsAg and HBeAg in HepG22215 cells. A combination of drug and ASODN could enhance the inhibition. Conclusion These results suggest that S-as ODN may be a potential new therapy for anti-HBV. The inhibition showed sequence-specific, dose-dependent and drug-cooperative effects.

Key concepts: HBeAg, HBsAg, Virology, Cell culture, Biology, Genome, Gene, Molecular biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Inhibition of HBsAg and HBeAg Expression by ASODN of Various Regions of the HBV Genome — Research Paper | ScholarLens