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2011•Chinese Journal of Clinical Rational Drug UseRequires access

Determination of the content of hypoxanthine and guanosine in inosine injection by HPLC

Wen Hong-me

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Abstract

Objective To establish a method of determining hypoxanthine and guanosine in inosine injection by HPLC.Methods Using Shiseido Superiorex ODS column(4.6mm×250mm,5μm);considering the methanol-water(10:90) as mobile phase,the flow rate was 1.0ml/min,UV detection was 248nm.Results The linear of hypoxanthine was 0.05~5μg/ml,r=0.9999,the limit of detection was 8.5ng;The linear of guanosine was 0.05~5μg/ml,r=0.9998,the limit of detection was 14.2ng.Conclusion The peak of related substances inosine can be separated effectively.Moreover,the method of operation is so simple,accurate,stable,specific.It can be used to determine the related substances of inosine injection.

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What this paper is about

Objective To establish a method of determining hypoxanthine and guanosine in inosine injection by HPLC.Methods Using Shiseido Superiorex ODS column(4.6mm×250mm,5μm);considering the methanol-water(10:90) as mobile phase,the flow rate was 1.0ml/min,UV detection was 248nm.Results The linear of hypoxanthine was 0.05~5μg/ml,r=0.9999,the limit of detection was 8.5ng;The linear of guanosine was 0.05~5μg/ml,r=0.9998,the limit of detection was 14.2ng.Conclusion The peak of related substances inosine can be separated effectively.Moreover,the method of operation is so simple,accurate,stable,specific.It can be used to determine the related substances of inosine injection.

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Available abstract

Objective To establish a method of determining hypoxanthine and guanosine in inosine injection by HPLC.Methods Using Shiseido Superiorex ODS column(4.6mm×250mm,5μm);considering the methanol-water(10:90) as mobile phase,the flow rate was 1.0ml/min,UV detection was 248nm.Results The linear of hypoxanthine was 0.05~5μg/ml,r=0.9999,the limit of detection was 8.5ng;The linear of guanosine was 0.05~5μg/ml,r=0.9998,the limit of detection was 14.2ng.Conclusion The peak of related substances inosine can be separated effectively.Moreover,the method of operation is so simple,accurate,stable,specific.It can be used to determine the related substances of inosine injection.

Key concepts: Inosine, Hypoxanthine, Guanosine, High-performance liquid chromatography, Detection limit, Medicine, Chromatography, Biochemistry

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