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2010•Zhongguo laonianxue zazhiRequires access

Relationship between multi-drug resistance relative protein-3 and liver caner multi-drug resistance

Guo Xiao

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Abstract

Objective To explore the relationship between the expression of multi-drug resistance relative protein-3 (MRP-3) and liver cancer multi-drug resistance.Methods The expression of MRP-3 in normal hepatocyte L-02,hepatocarcinoma cell BEL and hepatocarcinoma multi-drug resistance cell line HepG2/ADM were detected by RT-PCR.Anti-and oligonucleotide of MRP-3 were transfected by lipidosome into HepG2/ADM,intra-cellular doxorubicin (ADM) fluorescence intensity,resistance index,and the expression of MRP-3 mRNA were detected by MTT,flow cytometry,and RT-PCR.Results The expression of MRP-3 in HepG2/ADM was higher than that of normal hepatocyte L-02 and hepatocarcinoma cell BEL.After transfected with anti-oligonucleotide MRP-3,ADM fluorescence intensity,resistance index,and the expression of MRP-3 mRNA were all obviously decreased (P0.05).Conclusions Higher expression of MRP-3 maybe one of the mechanisms of hepatocarcinoma multi-drug resistance.

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What this paper is about

Objective To explore the relationship between the expression of multi-drug resistance relative protein-3 (MRP-3) and liver cancer multi-drug resistance.Methods The expression of MRP-3 in normal hepatocyte L-02,hepatocarcinoma cell BEL and hepatocarcinoma multi-drug resistance cell line HepG2/ADM were detected by RT-PCR.Anti-and oligonucleotide of MRP-3 were transfected by lipidosome into HepG2/ADM,intra-cellular doxorubicin (ADM) fluorescence intensity,resistance index,and the expression of MRP-3 mRNA were detected by MTT,flow cytometry,and RT-PCR.Results The expression of MRP-3 in HepG2/ADM was higher than that of normal hepatocyte L-02 and hepatocarcinoma cell BEL.After transfected with anti-oligonucleotide MRP-3,ADM fluorescence intensity,resistance index,and the expression of MRP-3 mRNA were all obviously decreased (P0.05).Conclusions Higher expression of MRP-3 maybe one of the mechanisms of hepatocarcinoma multi-drug resistance.

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Available abstract

Objective To explore the relationship between the expression of multi-drug resistance relative protein-3 (MRP-3) and liver cancer multi-drug resistance.Methods The expression of MRP-3 in normal hepatocyte L-02,hepatocarcinoma cell BEL and hepatocarcinoma multi-drug resistance cell line HepG2/ADM were detected by RT-PCR.Anti-and oligonucleotide of MRP-3 were transfected by lipidosome into HepG2/ADM,intra-cellular doxorubicin (ADM) fluorescence intensity,resistance index,and the expression of MRP-3 mRNA were detected by MTT,flow cytometry,and RT-PCR.Results The expression of MRP-3 in HepG2/ADM was higher than that of normal hepatocyte L-02 and hepatocarcinoma cell BEL.After transfected with anti-oligonucleotide MRP-3,ADM fluorescence intensity,resistance index,and the expression of MRP-3 mRNA were all obviously decreased (P0.05).Conclusions Higher expression of MRP-3 maybe one of the mechanisms of hepatocarcinoma multi-drug resistance.

Key concepts: Flow cytometry, Drug resistance, Transfection, Molecular biology, Hepatocyte, Messenger RNA, Drug, Cell culture

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Relationship between multi-drug resistance relative protein-3 and liver caner multi-drug resistance — Research Paper | ScholarLens