Effect of subeschar tissue fluid from burn patients on the expression of CD11/CD18 on PMN in vitro

Objective To investigate the effect of changes in CD11/CD18 expressions on polymorphonuclear neutrophils (PMNs) in response to subeschar tissue fluid (STF) obtained from burn patients. Methods The expression of CD11a/CD18 on the membrane of STF-treated PMN from normal subjects and CD11b/CD18 were determined by flow cytometry, and the activity of myeloperoxidase (MPO) in the media was determined. Results In response to STF stimulation, the expression of CD11a/CD18 and CD11b/CD18 on the membrane of PMN increased, reaching its peak at 1 h, and maintained during the following 24 h. The activity of MPO in the media underwent almost the same process. Conclusion STF can activate the PMNs and stimulate the expression of CD11/CD18 on the membrane of PMNs.