2011•Chinese Journal of DisinfectionRequires access

OBSERVATION ON APPLICATION AND STERILIZATION EFFICACY OF A DESKTOP AUTOCLAVE

Shibo Tang

Abstract

Objective To observe the application and sterilization efficacy of desktop autoclave in special medical articles sterilizing.Methods The sterilization efficacy of the desktop mini autoclave to cell culturing preparations was observed by chemical indicators.Results A desired sterilizing performance of the desktop autoclave with an internal steam source was observed in the sterilization of cell culturing preparations at 121℃ for 25 minutes and of surgical instruments,glass wares and rubber stoppers at 132℃ for 12 minutes.The wares and preparations sterilized by the autoclave did not affect the normal growth of cell cultures.Conclusion This desktop autoclave is applicable and effective in the sterilization of the routine wares and preparations used in cell culturing of ophthalmology laboratory.

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Key concepts: Sterilization (economics), Autoclave, Pulp and paper industry, Chemistry, Materials science, Metallurgy, Engineering, Foreign exchange market