5-Fluorouracil upregulates functional Fas ligand protein expression in colon cancer cells

Objective To investigate the regulatory effect of 5 fluorouracil on functional FasL protein expression in colon cancer cells.Methods By using Western blotting assay,FasL protein expression was exaimed in human colon cancer cell line SW620 with the administration of 5 fluorouracil in the concentration of 130?μmol/L,2?630?μmol/L and 400?μmol/L.Apoptosis of lymphocytes induced by FasL bearing tumor cells was determined by flow cytometry analysis.Result 5 fluorouracil can significantly upregulate FasL protein expression 2.64 to 4.08 folds in colon cancer cells.After treated with the drugs,tumor cells enhanced the ability to induce Jurkat cells apoptosis.Conclusion 5 fluorouracil can upregulate functional FasL protein expression in colon cancer and induce Jurkat cells apoptosis.