Effects of paeonol on the proliferation and apoptosis in human melanoma cell line A375 and M14

Objective:To determine the effects of paeonol on the apoptosis and proliferation in human melanoma cell line A375 and M14.Methods;The anti-proliferative activity of paeonol in A375 and M14 cells was investigated by CCK8 assay.After A375 and M14 cells were treated by 1.25 mmol/L,2.5 mmol/L or 5 mmol/L paeonol for 24 h,flow cytometry was used to detect the cell cycle and Annexin V-FITC and propidium iodide(PI) double staining was taken to detect the apoptosis rate of A375 and M14 cells,respectively.The morphological changes of A375 and M14 cells treated by 5 mmol/L paeonol for 24 h were observed by Hoechest 33258 staining.Results;Compared with untreated A375 and M14 cells,an increase of cell population in G2/M phase was observed in paeonol- treated cells.After treatment of 0 mmol/L,1.25 mmol/L,2.5mmol/L and 5 mmol/L paeonol for 24 h,the early apoptosis rate of A375 cells was 3.11%±0.53%,13.74%±1.73%,25.95%±0.57%and 46.44%±0.81%,respectively,while the rate of M14 cells was 1.00%±0.08%,2.00%±0.01%,2.99%±0.29%and 14.73%±0.94%,in a dose-dependent manner.After treated with paeonol of 5 mmol/L,there were morphological changes of apoptosis in A375 and M14 cells.Conclusion;Paeonol can inhibite proliferation and induce apoptosis of human melanoma cell line A375 and M14.