ScholarLens Back to search
2008•Journal of Xinjiang Agricultural UniversityRequires access

Construction and Expression of Recombinant Antigen P32 Gene of Goatpox Virus

Xuepeng Cai

Open publisher page 0 citations

Abstract

The structural protein P32 gene of the goat pox virus in the recombinant plasmid pMD18-P32 was subcloned into the Pichia pastoris expression vector pPIC9K.The resultant recombinant plasmid pPIC9K-P32 was transformed into P.pastoris GS115 by electroporation.The multi-copy recombinant P.pastoris strains were screened by G418 and induced by methanol.The expressed product was analyzed by SDS-PAGE and Western-blotting tests.The results indicated that the recombinant protein was expressed in P.pastoris,and had biological activity,the protein could be recognized by anti-serum against goat pox virus.

About this research paper

What this paper is about

The structural protein P32 gene of the goat pox virus in the recombinant plasmid pMD18-P32 was subcloned into the Pichia pastoris expression vector pPIC9K.The resultant recombinant plasmid pPIC9K-P32 was transformed into P.pastoris GS115 by electroporation.The multi-copy recombinant P.pastoris strains were screened by G418 and induced by methanol.The expressed product was analyzed by SDS-PAGE and Western-blotting tests.The results indicated that the recombinant protein was expressed in P.pastoris,and had biological activity,the protein could be recognized by anti-serum against goat pox virus.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The structural protein P32 gene of the goat pox virus in the recombinant plasmid pMD18-P32 was subcloned into the Pichia pastoris expression vector pPIC9K.The resultant recombinant plasmid pPIC9K-P32 was transformed into P.pastoris GS115 by electroporation.The multi-copy recombinant P.pastoris strains were screened by G418 and induced by methanol.The expressed product was analyzed by SDS-PAGE and Western-blotting tests.The results indicated that the recombinant protein was expressed in P.pastoris,and had biological activity,the protein could be recognized by anti-serum against goat pox virus.

Key concepts: Pichia pastoris, Recombinant DNA, Electroporation, Molecular biology, Plasmid, Biology, Virology, Expression vector

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Construction and Expression of Recombinant Antigen P32 Gene of Goatpox Virus — Research Paper | ScholarLens