The Expression and Role of NF-κβ on Neuron Injury Following Complete Cerebral Ischemia/Reperfusion in Rat Hippocampus

Objective To investigate whether NF-κβ may be involve in neuron injury and what is the mechanism it may play after complete cerebral ischemia/reperfusion.Methods A model of rats with four vessels occluded which induced global ischemia was used in this study.Male wister rats were divided randomly into three group.(Ⅰ) Sham-operated control group.(Ⅱ) Cerebral isehemia and (Ⅲ) cerebral ischemia group treated with PDTC(PDTC group).We observed the chan- ges of NF-κβ activity,TNF-α content and brain edema at several phasic points following cerebral ischemia/reperfusion.The treatment of PDTC(Ⅲ) on above changes was observed also.Results The NF-κβ activity,TNF-α content in hippocampus increased apparently,and the brain edema also increased apparently(P0.05 and P0.01),in PDTC-treating group,the abnormal change indeses as above were ame-lorated markedly,and there was significant difference between IR group and PDTC group(P0.05 and P0.01).Conclusion With active TNF-α content increasing,NF-κβ activity of hippocam- pus after ischemia/reperfusion increased,the brain edema increased also,all the changes decreased apparently with treat- ment of PDTC,which suggested that NF-κβ might get involved in neuron injury after ischemia/reperfusion.