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2008•Guangxi Medical JournalRequires access

Effect of microwave hyperthermia on drug-resistance of MCF-7 cells

Wenxian Zhou

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Abstract

Objective To study the effect of microwave hyperthermia on expression of multidrug resistance related(MDR)protein and intracellular ADM concentration of MCF-7/ADM cells.Methods Breast cell line MCF-7 and its ADM-resistance cell line MCF-7/ADM were treated with ADM and microwave hyperthermia.The levels of cellular multidrug resistance related protein(MRP),P-glycoprotein(P-gp)were detected by Real-Time PCR.The intracellular concentration of adriamycin was measured by high performance liquid chromatography.Results Protein levels of MRP and P-gp in MCF-7/ADM cells were higher than that in MCF-7 cells,which were markedly decreased in MCF-7/ADM cells after microwave hyperthermia.The concentration of adriamycin in MCF-7/ADM cells was significantly increased after microwave hyperthermia.Conclusion Microwave hyperthermia inhibited the expression of multidrug resistance related(MDR)proteins and increased the intracellular ADM concentration.The results suggest that microwave hyperthenmia could be used as a method to overcome MDR.

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Objective To study the effect of microwave hyperthermia on expression of multidrug resistance related(MDR)protein and intracellular ADM concentration of MCF-7/ADM cells.Methods Breast cell line MCF-7 and its ADM-resistance cell line MCF-7/ADM were treated with ADM and microwave hyperthermia.The levels of cellular multidrug resistance related protein(MRP),P-glycoprotein(P-gp)were detected by Real-Time PCR.The intracellular concentration of adriamycin was measured by high performance liquid chromatography.Results Protein levels of MRP and P-gp in MCF-7/ADM cells were higher than that in MCF-7 cells,which were markedly decreased in MCF-7/ADM cells after microwave hyperthermia.The concentration of adriamycin in MCF-7/ADM cells was significantly increased after microwave hyperthermia.Conclusion Microwave hyperthermia inhibited the expression of multidrug resistance related(MDR)proteins and increased the intracellular ADM concentration.The results suggest that microwave hyperthenmia could be used as a method to overcome MDR.

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Available abstract

Objective To study the effect of microwave hyperthermia on expression of multidrug resistance related(MDR)protein and intracellular ADM concentration of MCF-7/ADM cells.Methods Breast cell line MCF-7 and its ADM-resistance cell line MCF-7/ADM were treated with ADM and microwave hyperthermia.The levels of cellular multidrug resistance related protein(MRP),P-glycoprotein(P-gp)were detected by Real-Time PCR.The intracellular concentration of adriamycin was measured by high performance liquid chromatography.Results Protein levels of MRP and P-gp in MCF-7/ADM cells were higher than that in MCF-7 cells,which were markedly decreased in MCF-7/ADM cells after microwave hyperthermia.The concentration of adriamycin in MCF-7/ADM cells was significantly increased after microwave hyperthermia.Conclusion Microwave hyperthermia inhibited the expression of multidrug resistance related(MDR)proteins and increased the intracellular ADM concentration.The results suggest that microwave hyperthenmia could be used as a method to overcome MDR.

Key concepts: Hyperthermia, Intracellular, Multiple drug resistance, Cell culture, MCF-7, Chemistry, P-glycoprotein, Microwave

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