Effects of Single-embryo Vitrification on the Cleavage Stage Embryos Developmental Ability in vitro

Objective To compare the effect between single-embryo vitrification and slow-freezing on development of mice cleavage stage embryos in vitro,and to explore whether that vitrification is feasible to freeze preserve a great quantity of embryos.Methods Mice 8-cell embryos were cryopreserved by vitrification or slow-freezing randomly and cultured until the blastocyst hatched out after thawed.The embryo recovery rate,blastomere mortality,blastocyst formation rate,the number of cell clusters,hatched rate were counted and statistically analyzed.Results In the vitrification group,the recovery rate,blastomere mortality,blastocyst formation rate,hatched rate,the number of cell clusters were 100%,12.7%,75.7%,66.4%,112,respectively.While they were 87.1%,31.0%,35.5%,58.7%,78 in slow freezing group,respectively.All these indexes were significantly difference between two groups excluding the hatched rate.Conclusion The survival rate of mice cleavage stage embryos cryopreserved by single-embryo vitrification were high,the structure integrality and developmental abillity of embryos could be better maintained by this method.