Effect of 5-aza-2'-deoxycytidine on the expression of Gadd45α and apoptotic of A431 cells

Objective To study the effect of 5-aza-2'-deoxycytidine(5-Aza-dC) on the expression of Gadd45α and apoptosis of A431 cells.Methods Reverse transcription PCR,Western blot and immunocytochemistry were used to detect Gadd45α mRNA and protein expressions in A431 cells after treatment with 10 μmol/L 5-aza-dC for 48 hours,and flow cytometry was used to measure the cell apoptosis rate of A431 cells.Results After treatment with 10 μmol/L 5-aza-dC for 48 hours,Gadd45α expression of A431 cells at both mRNA and protein levels,the early apoptosis rate,late apoptosis rate and total apoptosis rate were significantly higher than those in untreated group(P0.05).Conclusion DNA methylation may be involved in the modulation of aberrant Gadd45α gene expression in A431 cells,and 5-aza-dC could induce the apoptosis of A431 cells.