Hydroquinone Induced Down-regulated Expression of PARP-1 and miR-221 in TK6 cells

Objective To explore the mechanism of decreased PARP-1 expression induce by hydroquinone.Methods Hydroquinone dissolved with PBS buffer,2.5,5.0 and 10.0 μmol/L hydroquinone were given to the TK6 cells,respectively,and the cells treated with PBS as the control.MiR-221 expression was defined by reverse transcription real-time RT-PCR assay,PARP-1 protein expression was detected by immunoblotting,and potential target gene for miR-221 was analyzed by using bioinformatics methods.Results The expression of PARP-1 protein shown a negative association with the doses of hydroquinone,miR-221 expression were inhibited by 0.17-,0.42-fold by 5.0 and 10.0 μmol/L hydroquinone,respectively.A regulatory element complex was observed between miR-221 and 3'untranslated region(UTR) of MBD2 mRNA.Conclusion PARP-1 inactivation induced by hydroquinone may be associate with the down-regulated expression of miR-221.