Study on suppressing-tumor effect of WWOX gene transfection

Objective To construct tumor suppressor gene WWOX eukaryotic expression vector and observe the apoptotic effect of WWOX transient transfer on A549 human lung adenocarcinoma cells.Methods Tumor suppressor gene WWOX was cloned by RT-PCR and then linked into pMD18-T vector.After sequenced,the expression vector pcDNA4/myc-WWOX was constructed and transiently transfected into A549 cells under mediation of KeyGenTransⅠin vitro.The expression of WWOX was detected by RT-PCR and Western blot.The apoptosis of transfected cells was analyzed by flow cytometry.The cell survival level was determined through clone formation assay.Results The expression vector pcDNA4/myc-WWOX was successfully constructed.The percentage of apoptotic cells in transfected pcDNA4/myc-WWOX cells was significantly increased compared with that in non-transfected and pcDNA4/myc-His transfected cells.The rate of clone formation of pcDNA4/myc-WWOX was lower than that in non-transfected and pcDNA4/myc-His tansfected cells.Conclusions Transiently transfecting expression vector pcDNA4/myc-WWOX into tumor cells can induce apoptosis and obviously inhibit cell survival.