Determination of 1-Deoxynojimycin Contents in Leaves of Mulberry Cultivars in Shandong Province

In order to select mulberry cultivars suitable for development of medicinal and healthcare product prepared with mulberry leaves,the contents of 1-deoxynojimycin(DNJ) in leaves of representative 59 mulberry varieties cultivated in sericultural areas of Shandong Province in spring were determined by reversed-phase high performance liquid chromatography(RP-HPLC).The results indicated that the contents of DNJ in different mulberry cultivars had extremely significant difference.The ratio between maximum and minimum DNJ content can reach 3.2.DNJ contents in leaves of Yiyuanhuanglu of Morus multicaulis Perr.and Hongmanao of Morus alba Linn.were relatively higher,with mass fraction as high as 0.409 7% and 0.382 8% respectively.In comprehensive consideration of DNJ content in mulberry leaves,annual mulberry leaf yield per unit area of mulberry field and resistance against environmental stresses,Qinghuangsang and Xuan 792 had higher practical value,being appropriate special mulberry varieties or breeding materials for development of mulberry leaf DNJ.