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Subcloning of human immunodeficiency virus type I genes

Ji Chang

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Abstract

We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. Digested with Kpn I, the recombinant plasmid pARV-2/7A was cleaved into 4 fragments. The 6.0 kb fragment was recircularized, and the other three fragments were ligated with pUC19 plasmid cut open by Kpn I. By screening and identification, four subclones were obtained. Of which, pJE332 contains the whole env gene, pJG423 contains LTR, gag and part of pol gene, pJP163 and pJP032 contain only part of pol gene. These subclone plasmids have been being used in many investigations on gene detection and cloning of HIV type I.

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What this paper is about

We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. Digested with Kpn I, the recombinant plasmid pARV-2/7A was cleaved into 4 fragments. The 6.0 kb fragment was recircularized, and the other three fragments were ligated with pUC19 plasmid cut open by Kpn I. By screening and identification, four subclones were obtained. Of which, pJE332 contains the whole env gene, pJG423 contains LTR, gag and part of pol gene, pJP163 and pJP032 contain only part of pol gene. These subclone plasmids have been being used in many investigations on gene detection and cloning of HIV type I.

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Available abstract

We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. Digested with Kpn I, the recombinant plasmid pARV-2/7A was cleaved into 4 fragments. The 6.0 kb fragment was recircularized, and the other three fragments were ligated with pUC19 plasmid cut open by Kpn I. By screening and identification, four subclones were obtained. Of which, pJE332 contains the whole env gene, pJG423 contains LTR, gag and part of pol gene, pJP163 and pJP032 contain only part of pol gene. These subclone plasmids have been being used in many investigations on gene detection and cloning of HIV type I.

Key concepts: Subcloning, pUC19, Plasmid, Molecular biology, Biology, Gene, Complementary DNA, Virology

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