Influences of lipopolysaccharide on the inflammation and lipid metabolism in rabbits induced by high cholesterol diet

Objective To study the influences of lipopolysaccharide on inflammation and lipid metabolism in rabbits induced by high cholesterol diet.Methods Rabbits were randomized to three groups.Complex model group were fed with feed containing 0.5% cholesterol for 3 weeks.At the fourth,eighth and twelfth weeks,rabbits were given injection of lipopolysaccharide(1 μg) at auricular artery,cervical part(i.m.) and fold inguen(i.m.),respectively.Normal group were fed with normal diet,while High cholesterol group were fed with feed confaining 0.5% cholesterol only.After 16 weeks,vascular tissues were analyzed using morphometric and histological methods,and the sera were checked for cholesterol,LDL-C,C-reaction protein and tumor necrosis factor-α(TNF-α).Results The cholesterol and LDL-C of High cholesterol group were increased,compared to those of normal group.The cholesterol and LDL-C of Complex medel group were obviously higher than those of High cholesterol group.The TNF-α of High cholesterol group were higher than that of noamal group,while the TNF-α of Complex model group were higher than that of High cholesterol group.Pathological results showed obvious changes in aortic arch.In Comlex model group the endangium plaque were widespread,while in that of High cholesterol group there was only single small plaque.The pathological heart changes of both High cholesterol group and Complex model group weren′t obvious,with mild oedema and small fat drops.The liver cells of High cholesterol group showed mild oedema,while fat drops in the liver of Complex model group were more typical.Conclusion LPS intensifies the formation of vascular plaques and aggravates the lipometabolic disorder and inflammation.