Establishment of oxygen-glucose deprivation model of rats′ cortex and hippocampal neurons in vitro

Objective To establish oxygen-glucose deprivation model of rats′ cortex and hippocampusal neurons in vitro.Methods The cortex and hippocampal neurons cultured for 10～12 days were exposed to Earle′s solution with sodium dithionite(Na_2S_2O_4)(1 mmol/L) and free glucose in stand of original culture medium containing serum for 2,4,8,12,24 and 36 h respectively.3-(4,5-dimethylthiazol-2yl)-2,5diphenylterazolium bromide(MTT)assay was applied to evaluate the neuronal vigor.Biochemical method was used to determine lactate dehydrogenase(LDH)activity.Results The rat cortex and hippocampal neurons vigor significantly decreased in a time-dependent manner cultured by Earle′s solution with sodium dithionite 1 mmol/L and free glucose for 2～36 h(P0.01),and the neuron survival rate decreased to 25.4% and 24.2% respectively 36 h later.However,the LDH activity significantly increased in a time-dependent manner(P0.01)showing great negative correlation to neurons vigor(r=-0.983,-0.992,P0.01).Conclusions The oxygen-glucose deprivation model of rats′ cortex and hippocampal neurons in vitro could be established successfully by Earle′s solution with sodium dithionite 1 mmol/L and free glucose.