Rapid Construction of GPR126 Conditional Gene-targeting Vector

Generation of specific gene knockout mouse model is a reliable technique for studying the role of genes in mammalian model organism.One of the key steps to acquire a gene knockout mouse is to construct a targeting vector for homologous recombination in mouse embryonic stem cells.For some genes,the mutants will die in uteri owing to their critical roles in embryonic development,or the mutant mice may have different phenotypes according to the period of development and types of tissues.It is difficult to study these genes by the conventional knockout approaches.Thereby the conditional knockout strategy had been developed for its advantages to circumvent the embryonic lethality problem and to investigate gene function temporally and spatially.Using modified Red homologous recombineering system,the two LoxP sites were inserted into the target position accurately and the GPR126 conditional gene-targeting vector was rapidly constructed.The successful generation of GPR126 conditional knockout construct will be helpful for the subsequent production of knockout mouse model and exploration of the function of GPR126 in mice.