Optimization of Preparation Process of Soybean Peptide Using Sequential Design

Hydrolysis process of soybean protein by Alcalase protease under the condition of uncontrolled pH was investigated.The effects of the main parameters including enzyme/substrate ratio,soybean protein concentration,initial pH,hydrolysis time,and hydrolysis temperature were analyzed and the hydrolysis process was optimized using sequential design.After optimization,the optimum preparation process of soybean peptide was deter mined: soybean protein concentration 4.92 %,enzyme/subst rate ratio 2 758 kat/g,initial pH11.0,hydrolysis temperature 55 ℃ and hydrolysis time 7 h.Under these optimized conditions,hydrolysis degree value of soybean protein could reach 39.10mg/g,and the yield of polypeptides was 63.21 %.