Expression and Significance of CD4~+ CD25~+ Treg,FOXP3 Gene in the Myelodysplastic Syndrome

Objective To explore the expression levels and the clinic significance of CD4+CD25+ regulatory T cell (CD4+ CD25+ Treg cell) and regulator and control gene FOXP3 in the myelodysplastic syndrome (MDS) patients.Methods The expression levels of CD4+ CD25+ Treg was detected by means of immunofluorescence and flow cytometry in 24 patients with MDS [MDS group:divide by the standard of MDS grouping:refractory anemia group (RA group) 6 examples,refractory cytopenia with multilineage dysplasia group (RCMD group) 11 examples,refractory anemia with excess blasts group(RAEB group) 5 examples] and 8 normal controls,respectively,that of FOXP3 by real-time quantitative RT-PCR.Results The expression levels of CD4+ CD25+ Treg and FOXP3 in the MDS group were not obviously different with the normal control group(all P0.05);patients with RA group and RAEB group,The expression levels of CD4+ CD25+ Treg and FOXP3 were obviously different with the normal control group (all P0.01);The expression levels of CD4+ CD25+ Treg and FOXP3 in the RCMD group were not obviously different with the normal control group (all P0.05);patients with RCMD group and RAEB group,The expression levels of CD4+ CD25+ Treg and FOXP3 were obviously different with the RA group(all P0.01);patients with RA group and RCMD group,The expression levels of CD4+ CD25+ Treg and FOXP3 were obviously different with the RAEB group(all P0.01).Conclusion The percentage of CD4+ CD25+ Treg in CD4+T cell and the expression level of FOXP3 depends on the risk category of MDS;higher risk indicates more percentages and expressions,suggesting that immunologic abnormality is a promoting factor in the onset and progression.