Determination and dynamic analysis of α-pinene and β-pinene in volatile oil of Myristica fragrans Houtt

OBJECTIVE To establish a GC method for simultaneous determination of α-pinene and β-pinene in volatile fractions and study its dynamic changes in leaves of Myristica fragrans Houtt;METHODS The essential oil was extracted by steam distillation.GC method was applied for the determination of α-pinene and β-pinene from the essential oil.Chromatographic conditions:HP-5MS Capillary column(0.25mm×30m×0.25μm) was used;The detector was FID;Inlet temperature was 240℃;The detector temperature was 250℃;Initial temperature was 40℃.It was programmed rising by 5℃·min-1 until 220℃.Carrying gas was nitrogen(1mL·min-1),split ratio was 10:1;Injecton volume was 1μL.RESULTS α-pinene and β-pinene have good linear relationship in the concentration range with the average recoveries(n=3) were 99.2% and 98.7%,respectively.The contents of α-pinene and β-pinene in different tissues were different.The content of α-pinene and βpinene in the leaves from different months showed a certain degree of regularity.CONCLUSION This method is simple,quick and specific.It can be applied for the simultaneous determination of α-pinene and β-pinene in the volatile fractions of Myristica fragrans Houtt.