An Analysis on Differential Expression of Hemolymph Proteins Between the Normal and Moltinism Altered Larvae of Bombyx mori Before and After the Last Molting

Moltinism of the silkworm(Bombyx mori)is not only controlled by major genes on its chromosomes but also regulated by genes associated with sex maturity and influenced by environmental factors as well.In order to explore the molecular mechanism of silkworm moltinism alteration induced by environmental factors,two silkworm tetramolter varieties,898 huanglu and D92 huanglu,and one trimolter variety,Sanmian A,were used as the materials to induce moltinism alternation through controlling the factors including temperature,humidity and nutrient supply in egg incubation stage or during young larva stage.Through two-dimensional electrophoresis and matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-TOF),the differential expression of hemolymph proteins between the normal and moltinism altered larvae of the silkworm before and after the last molting were analyzed.As a result,totally 13 proteins,including transferrin,chymotrypsin inhibitor SCI-I,polyadenylate binding protein 2,27 kD glycoprotein precursor,ribosomal protein L20 and mitochondrial ribosomal protein L2,were found to be up-or downregulated,showing that they are involved in moltinism alteration of silkworm.The obtained data comprise a good basis for further elucidation on the mechanism of silkworm moltinism alteration at protein level.